This calculator helps you estimate the anion gap based on sodium (Na⁺), chloride (Cl⁻), and bicarbonate (HCO₃⁻) levels measured in blood serum.
advanced mode, there is an option to input potassium (K⁺) and albumin levels to get a more accurate result or to see the anion gap corrected for albumin.
Read on to understand the anion gap calculation, to learn what is anion gap and what is a normal anion gap is.
🙋 Please note that this calculator estimates the anion gap for serum anions. Check out our urine anion gap calculator for urine anion gap.
What is anion gap?
In blood serum, the total number of cations (positive ions) should equal the total number of anions (negative ions), so the overall electrical charge is neutral.
However, routine tests do not measure all types of ions. The anion gap is the difference between the measured cations and anions. It represents how many ions are not accounted for by the lab measurements.
In medicine, anion gap calculation is required when attempting to identify the cause of acidosis, which is a lower than normal pH in the blood. It is usually calculated from sodium, chloride, and bicarbonate concentrations in the blood serum.
How is the anion gap calculated?
To estimate the anion gap, measuring electrolyte levels in the blood serum is necessary.
It is the difference between the sodium (Na⁺) cations and chloride (Cl⁻) and bicarbonate (HCO₃⁻) anions:
AG = [Na⁺] − ([Cl⁻] + [HCO₃⁻])
Potassium concentrations are very low compared to other ions, so they're omitted from the standard calculation. You can find the result is more accurate when including potassium, though. Then, the equation looks like this:
AG = [Na⁺] + [K⁺] − ([Cl⁻] + [HCO₃⁻])
What is a normal anion gap?
The reference range when potassium is omitted is 3-11 mEq/L. When including potassium in the equation, it's 5-16 mEq/L.
Interpretation of the outcome:
High anion gap >11 mEq/L (or >16 mEq/L with potassium):
A high anion gap indicates high anion gap acidosis, for example, lactic acidosis or ketoacidosis.
Normal anion gap 3-11 mEq/L (or 5-16 mEq/L with potassium):
In patients with regular anion gap acidosis, the primary pathology is a drop in HCO₃⁻ and an increase of Cl⁻. It is called hyperchloremic acidosis.
Low anion gap - <3 mEq/L (or <5 mEq/L with potassium):
Hypoalbuminemia frequently causes a low anion gap.
Why is anion gap corrected for albumin?
Low albumin levels (hypoalbuminemia), common in critically ill patients, can mask a mild elevation of the anion gap. For the correct interpretation of the anion gap, it is crucial to correct it for serum albumin with the Figge-Jabor-Kazda-Fencl equation:
Corrected AG = AG + 2.5 × ([Normal Albumin] - [Measured Albumin])
If you're interested to know more about the correction for albumin, read this.